Comparative proteomics study of mitochondrial electron transport system modulation in SH-SY5Y cells following MPP+ versus 6-OHDA-induced neurodegeneration

Abstract Parkinson’s disease (PD) is the second-most common neurodegenerative worldwide. Several studies have investigated PD for decades; however, exact mechanism of development remains unknown. To study PD, SH-SY5Y cells are often treated with 6-hydroxydopamine (6-OHDA) or 1-methyl-4-phenylpyridinium (MPP+) to induce PD. understand pathogenesis, we confirmed protein changes between 6-OHDA- and MPP+-treated via proteomics analysis using liquid chromatography coupled tandem mass spectrometry. 6-OHDA-treated showed increased expression electron transporter-related proteins compared that in control group, along decreased cells. However, both down- upregulation mitochondrial dysfunction apoptosis. These were protein–protein interaction network IPA STRING Cell-based experiments flow cytometry verified apoptosis membrane potential Our results provide new insights into thereby contributing understanding mechanisms development.